Physicochemical Characterization and Determination of Free Radical Scavenging Activity of Rutin-phospholipid Complex

Rutin belongs to a class of plant secondary metabolites called flavonoids. Rutin is believed to be a vital nourishing supplement as it is able to make the capillaries stronger. But when administered orally it shows poor absorption because of less lipophilicity. To overcome this limitation, the present study was aimed to develop Rutinphospholipid complex in different ratio to improve the lipophilic properties of rutin. The prepared complex was evaluated for physicochemical properties and in-vitro DPPH radical’s scavenging activity. The physicochemical properties of the complex were analyzed by ultraviolet-visible spectroscopy (UV), infrared spectroscopy (IR) and X-ray diffractometry (XRD). The results showed that rutin-phospholipid in the complex were joined and did not form a new compound. Results of DPPH radical are scavenging activity revealed that among all ratios of complex, 1:3 next to this 1:2 followed by 1:1. The study was concluded that the free radical scavenging activity was observed in concentration dependent manner. INTRODUCTION: Rutin (3, 3′, 4′, 5, 7-pentahydroxyflavone-3-rhamnoglucoside) 1 is a flavanol glycoside (figure 1) plant metabolite extracted from buckwheat and Japanese pagoda tree and is able to antagonize the increase of capillary fragility associated with hemorrhagic disease or hypertension .